Correcting what was widely seen as an historic wrong, Beyoncé won best album at the 67th Grammy Awards in Los Angeles.
The star was recognised for her eighth album, Cowboy Carter, which celebrates and contextualises the black roots of country music. She had previously been passed over for the ceremony's main prize on four separate occasions.
As her name was read out, Beyoncé hugged her daughter Blue Ivy, then her husband Jay-Z, before walking to the stage in a gold paisley dress to accept the trophy.
"I just feel very full and very honoured," she said. "It's been many, many years."
The singer dedicated her award to Linda Martell, one of the pioneers for black musicians in country music and the first black woman to perform solo at the Grand Ole Opry (Ms Martell features on several of Cowboy Carter's songs).
"I hope we just keep pushing forward [and] opening doors," Beyoncé continued. "God bless y'all. Thank you so much."
The star's victory comes 25 years after her first Grammy nomination, as part of the R&B group Destiny's Child.
She went on to become the most-awarded artist in Grammys history, with a haul of 35 trophies - but the main prize eluded her until Sunday night.
In the end, it took a switch of genre to earn the Texas-born musician a win.
Focusing on country and Americana, Cowboy Carter is the second part of a trilogy of albums that interrogates American musical traditions and uncovers the often unheralded contributions made by black artists.
Notably, it makes Beyoncé the first black woman to win album of the year since 1999, when Lauryn Hill's The Miseducation of Lauryn Hill took the title.
